Whole Genome Sequencing Introduction to the Interpretation of Whole Genome Sequence Data in Food Safety
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چکیده
I ntroduction Whole genome sequencing (WGS) for bacterial foodborne pathogen characterization is here to stay. Developments in WGS platforms have made it possible to sequence the entire genome of bacteria at prices comparable to common molecular subtyping methods. These data can provide nearperfect discrimination of bacterial isolates. While common molecular subtyping methods only interrogate small parts of the genome (e.g., for pulsed-field gel electrophoresis [PFGE] restriction sites, MLST sequence of ~7 loci of ~500 basepairs [bp]), WGS approaches make it possible to interrogate more than 99% of the genome, which translates to approximately 2.8 million and 4.8 million basepairs in Listeria monocytogenes and Salmonella enterica, respectively.
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